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LOCUS NUWT_XGATE 8118 BP SS-DNA CIRCULAR SYN 20-NOV-2003 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers misc_feature 4011..>4169 /note="NubWT-B1" terminator 5756..5952 /note="ADH1 terminator" promoter 3650..4006 /note="ADH1 promoter" misc_feature 4171..5644 /note="KanMX cassette" misc_feature 5651..5707 /note="B2 linker" misc_feature 5708..5734 /note="HA tag" misc_feature 5651..5734 /note="B2-HA" BASE COUNT 2211 A 1765 C 1848 G 2294 T 0 OTHER ORIGIN - 1 GAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT 61 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT 121 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA 181 ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG 241 CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG 301 CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT 361 GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG 421 GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT 481 AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA 541 TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA 601 GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC 661 ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA 721 AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG 781 GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG 841 ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT 901 CCGGTGCGTT TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG 961 CTCTGAAGTT CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT 1021 TCCGAAAACG AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA 1081 CGTCGCACCT ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT 1141 GCGTGTTTAT GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC 1201 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT 1261 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC 1321 TATCATTTCC TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA 1381 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA 1441 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 1501 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA 1561 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAGA TCAACGACAT TACTATATAT 1621 ATAATATAGG AAGCATTTAA TAGACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC 1681 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA 1741 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAGAAT TAATTCGGTC GAAAAAAGAA 1801 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA 1861 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT 1921 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG 1981 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG 2041 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT 2101 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG 2161 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG 2221 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG 2281 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG 2341 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT 2401 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA 2461 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA 2521 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT 2581 TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA 2641 GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA 2701 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA 2761 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT 2821 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC 2881 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA 2941 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC 3001 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT 3061 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG 3121 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 3181 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 3241 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCGT CCAAGCTTTC GCGAGCTCGA 3301 TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT CTTTTCTGGC AACCAAACCC 3361 ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA ACATGTAGGT GGCGGAGGGG 3421 AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG CTAAACAAGA CTACACCAAT 3481 TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC TGTAGCCCTA GACTTGATAG 3541 CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG CCATCTATTG AAGTAATAAT 3601 AGGCGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC 3661 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA 3721 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC 3781 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT 3841 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA 3901 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT 3961 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCGAG ATGCAGATTT 4021 TCGTCAAGAC TTTGACCGGT AAAACCATAA CATTGGAAGT TGAATCTTCC GATACCATCG 4081 ACAACGTTAA GTCGAAAATT CAAGACAAGG AAGGAATCCC TGGTGGGTCG ACAAGTTTGT 4141 ACAAAAAAGC AGGCTCTCCA ACCACCATGA ATTCTATACG AAGTTATTAG GTCTAGAGAT 4201 CTGTTTAGCT TGCCTCGTCC CCGCCGGGTC ACCCGGCCAG CGACATGGAG GCCCAGAATA 4261 CCCTCCTTGA CAGTCTTGAC GTGCGCAGCT CAGGGGCATG ATGTGACTGT CGCCCGTACA 4321 TTTAGCCCAT ACATCCCCAT GTATAATCAT TTGCATCCAT ACATTTTGAT GGCCGCACGG 4381 CGCGAAGCAA AAATTACGGC TCCTCGCTGC AGACCTGCGA GCAGGGAAAC GCTCCCCTCA 4441 CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA GGTTAGGATT 4501 TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA TACAGTTCTC 4561 ACATCACATC CGAACATAAA CAACCATGGG TAAGGAAAAG ACTCACGTTT CGAGGCCGCG 4621 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 4681 GCAATCAGGT GCGACAATCT ATCGATTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 4741 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 4801 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 4861 ATGGTTACTC ACCACTGCGA TCCCCGGCAA AACAGCATTC CAGGTATTAG AAGAATATCC 4921 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 4981 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 5041 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 5101 TGTTGAACAA GTCTGGAAAG AAATGCATAA GCTTTTGCCA TTCTCACCGG ATTCAGTCGT 5161 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 5221 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 5281 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 5341 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGT 5401 ACTGACAATA AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT 5461 GTAGTTGTTC TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC 5521 ATCTGCCCAG ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA 5581 ATGCTGGTCG CTATACTGCT GTCGATTCGA TACTAACGCC GCCATCCAGT GTCGAAAACG 5641 AGCTCCCGGG TACCCAGCTT TCTTGTACAA AGTGGTTGGT GGTGGCGGAT CCGGTGGAGG 5701 TGGATCATAC CCATACGACG TTCCAGATTA TGCTTAACAG CCAAGCTAAT TCCGGGCGAA 5761 TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA GTGTATACAA 5821 ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTG TTCTTGAGTA ACTCTTTCCT 5881 GTAGGTCAGG TTGCTTTCTC AGGTATAGCA TGAGGTCGCT CTTATTGACC ACACCTCTAC 5941 CGGCATGCAA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG 6001 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA 6061 TGAGTGAGGT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC 6121 CTGTCGTGCC AGCTGGATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 6181 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 6241 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 6301 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 6361 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 6421 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 6481 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 6541 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 6601 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 6661 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 6721 GCCACTAGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 6781 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG 6841 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 6901 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 6961 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 7021 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 7081 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 7141 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 7201 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 7261 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 7321 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 7381 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 7441 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 7501 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 7561 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 7621 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 7681 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 7741 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 7801 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 7861 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 7921 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 7981 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 8041 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 8101 CCCCGAAAAG TGCCACCT //
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