LOCUS       X-NUWTGATE   8120 BP SS-DNA   CIRCULAR  SYN       20-NOV-2003

DEFINITION  -

ACCESSION   -

KEYWORDS    -

SOURCE      -

FEATURES             Location/Qualifiers

     misc_feature    4016..4054

                     /note="B1 linker"

     promoter        3650..4006

                     /note="ADH1 promoter"

     terminator      5758..5954

                     /note="ADH1 terminator"

     gene            1884..2558

                     /note="TRP1"

     misc_feature    4060..5529

                     /note="KanMX cassette"

     misc_feature    5536..5592

                     /note="B2 linker"

     misc_feature    5593..>5694

                     /note="NubWT"

     misc_feature    5704..5733

                     /note="HA tag"

     misc_feature    5536..>5733

                     /note="B2-NuWT-HA "

BASE COUNT     2212 A   1765 C   1846 G   2297 T      0 OTHER

ORIGIN      -

        1 GAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT

       61 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT

      121 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA

      181 ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG

      241 CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG

      301 CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT

      361 GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG

      421 GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT

      481 AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA

      541 TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA

      601 GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC

      661 ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA

      721 AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG

      781 GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG

      841 ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT

      901 CCGGTGCGTT TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG

      961 CTCTGAAGTT CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT

     1021 TCCGAAAACG AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA

     1081 CGTCGCACCT ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT

     1141 GCGTGTTTAT GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC

     1201 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT

     1261 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC

     1321 TATCATTTCC TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA

     1381 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA

     1441 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG

     1501 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA

     1561 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAGA TCAACGACAT TACTATATAT

     1621 ATAATATAGG AAGCATTTAA TAGACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC

     1681 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA

     1741 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAGAAT TAATTCGGTC GAAAAAAGAA

     1801 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA

     1861 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT

     1921 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG

     1981 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG

     2041 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT

     2101 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG

     2161 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG

     2221 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG

     2281 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG

     2341 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT

     2401 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA

     2461 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA

     2521 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT

     2581 TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA

     2641 GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA

     2701 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA

     2761 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT

     2821 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC

     2881 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA

     2941 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC

     3001 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT

     3061 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG

     3121 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT

     3181 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG

     3241 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCGT CCAAGCTTTC GCGAGCTCGA

     3301 TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT CTTTTCTGGC AACCAAACCC

     3361 ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA ACATGTAGGT GGCGGAGGGG

     3421 AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG CTAAACAAGA CTACACCAAT

     3481 TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC TGTAGCCCTA GACTTGATAG

     3541 CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG CCATCTATTG AAGTAATAAT

     3601 AGGCGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC

     3661 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA

     3721 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC

     3781 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT

     3841 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA

     3901 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT

     3961 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCATA TGTGAACAAG

     4021 TTTGTACAAA AAAGCAGGCT CTCCAACCAC CATGAATTCT ATACGAAGTT ATTAGGTCTA

     4081 GAGATCTGTT TAGCTTGCCT CGTCCCCGCC GGGTCACCCG GCCAGCGACA TGGAGGCCCA

     4141 GAATACCCTC CTTGACAGTC TTGACGTGCG CAGCTCAGGG GCATGATGTG ACTGTCGCCC

     4201 GTACATTTAG CCCATACATC CCCATGTATA ATCATTTGCA TCCATACATT TTGATGGCCG

     4261 CACGGCGCGA AGCAAAAATT ACGGCTCCTC GCTGCAGACC TGCGAGCAGG GAAACGCTCC

     4321 CCTCACAGAC GCGTTGAATT GTCCCCACGC CGCGCCCCTG TAGAGAAATA TAAAAGGTTA

     4381 GGATTTGCCA CTGAGGTTCT TCTTTCATAT ACTTCCTTTT AAAATCTTGC TAGGATACAG

     4441 TTCTCACATC ACATCCGAAC ATAAACAACC ATGGGTAAGG AAAAGACTCA CGTTTCGAGG

     4501 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT

     4561 GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG

     4621 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA

     4681 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT

     4741 GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA

     4801 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT

     4861 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG

     4921 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC

     4981 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA

     5041 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA

     5101 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA

     5161 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT

     5221 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA

     5281 TCAGTACTGA CAATAAAAAG ATTCTTGTTT TCAAGAACTT GTCATTTGTA TAGTTTTTTT

     5341 ATATTGTAGT TGTTCTATTT TAATCAAATG TTAGCGTGAT TTATATTTTT TTTCGCCTCG

     5401 ACATCATCTG CCCAGATGCG AAGTTAAGTG CGCAGAAAGT AATATCATGC GTCAATCGTA

     5461 TGTGAATGCT GGTCGCTATA CTGCTGTCGA TTCGATACTA ACGCCGCCAT CCAGTGTCGA

     5521 AAACGAGCTC CCGGGTACCC AGCTTTCTTG TACAAAGTGG TTGGTGGTGG CGGATCCGGT

     5581 GGAGGTGGAT CAATGCAGAT TTTCGTCAAG ACTTTGACCG GTAAAACCAT AACATTGGAA

     5641 GTTGAATCTT CCGATACCAT CGACAACGTT AAGTCGAAAA TTCAAGACAA GGAAGGAATC

     5701 CCTGGTTACC CATACGACGT TCCAGATTAT GCTTAGCTGC AGCCAAGCTA ATTCCGGGCG

     5761 AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT AAGTGTATAC

     5821 AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TGTTCTTGAG TAACTCTTTC

     5881 CTGTAGGTCA GGTTGCTTTC TCAGGTATAG CATGAGGTCG CTCTTATTGA CCACACCTCT

     5941 ACCGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC

     6001 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT

     6061 AATGAGTGAG GTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA

     6121 ACCTGTCGTG CCAGCTGGAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA

     6181 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC

     6241 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG

     6301 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT

     6361 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA

     6421 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT

     6481 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC

     6541 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG

     6601 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT

     6661 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG

     6721 CAGCCACTAG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA

     6781 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA

     6841 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG

     6901 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG

     6961 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG

     7021 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT

     7081 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT

     7141 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC

     7201 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA

     7261 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG

     7321 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT

     7381 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA

     7441 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT

     7501 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT

     7561 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG

     7621 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG

     7681 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG

     7741 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA

     7801 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT

     7861 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT

     7921 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT

     7981 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA

     8041 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT

     8101 TTCCCCGAAA AGTGCCACCT

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