LOCUS       XNgate21_3HA   8194 BP SS-DNA   CIRCULAR  SYN     19-NOV-2003

DEFINITION  -

ACCESSION   -

KEYWORDS    -

SOURCE      -

FEATURES             Location/Qualifiers

     misc_feature    4016..4054

                     /note="B1 linker"

     misc_feature    1..1348

                     /note="two micron origin of replication"

     gene            1884..2558

                     /note="TRP1"

     misc_feature    2609..3277

                     /note="f1 origin of replication"

     gene            complement(7204..8064)

                     /note="beta lactamase (AmpR)"

     misc_feature    6033..7204

                     /note="pUC origin of replication"

     misc_feature    4066..5529

                     /note="KanMX cassette of pUG6"

     misc_feature    5536..5592

                     /note="B2 linker"

     gene            5593..5694

                     /note="NubG"

     misc_feature    5707..5733

                     /note="1st HA tag"

     misc_feature    5770..5796

                     /note="3rd HA tag"

     misc_feature    5536..5796

                     /note="B2NubG3HA"

     terminator      5829..6030

                     /note="ADH1 terminator"

     promoter        3650..4006

                     /note="ADH1 promoter"

     misc_feature    5737..5763

                     /note="2nd HA tag"

BASE COUNT     2228 A   1785 C   1865 G   2316 T      0 OTHER

ORIGIN      -

        1 GAACGAAGCA TCTGTGCTTC ATTTTGTAGA ACAAAAATGC AACGCGAGAG CGCTAATTTT

       61 TCAAACAAAG AATCTGAGCT GCATTTTTAC AGAACAGAAA TGCAACGCGA AAGCGCTATT

      121 TTACCAACGA AGAATCTGTG CTTCATTTTT GTAAAACAAA AATGCAACGC GAGAGCGCTA

      181 ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA CGCGAGAGCG

      241 CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC ATCCCGAGAG

      301 CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC GCTCTATAAT

      361 GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA GGCTACTTTG

      421 GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT ACTGATTACT

      481 AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT TCTATACCGA

      541 TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT TCATTGGTCA

      601 GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG AAATGTTTAC

      661 ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT TTTTGTCTAA

      721 AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG CAAGTTCAAG

      781 GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA TAGCAAAGAG

      841 ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATA TTTTAGTAGC TCGTTACAGT

      901 CCGGTGCGTT TTTGGTTTTT TGAAAGTGCG TCTTCAGAGC GCTTTTGGTT TTCAAAAGCG

      961 CTCTGAAGTT CCTATACTTT CTAGAGAATA GGAACTTCGG AATAGGAACT TCAAAGCGTT

     1021 TCCGAAAACG AGCGCTTCCG AAAATGCAAC GCGAGCTGCG CACATACAGC TCACTGTTCA

     1081 CGTCGCACCT ATATCTGCGT GTTGCCTGTA TATATATATA CATGAGAAGA ACGGCATAGT

     1141 GCGTGTTTAT GCTTAAATGC GTACTTATAT GCGTCTATTT ATGTAGGATG AAAGGTAGTC

     1201 TAGTACCTCC TGTGATATTA TCCCATTCCA TGCGGGGTAT CGTATGCTTC CTTCAGCACT

     1261 ACCCTTTAGC TGTTCTATAT GCTGCCACTC CTCAATTGGA TTAGTCTCAT CCTTCAATGC

     1321 TATCATTTCC TTTGATATTG GATCATATTA AGAAACCATT ATTATCATGA CATTAACCTA

     1381 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA

     1441 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG

     1501 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA

     1561 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATAGA TCAACGACAT TACTATATAT

     1621 ATAATATAGG AAGCATTTAA TAGACAGCAT CGTAATATAT GTGTACTTTG CAGTTATGAC

     1681 GCCAGATGGC AGTAGTGGAA GATATTCTTT ATTGAAAAAT AGCTTGTCAC CTTACGTACA

     1741 ATCTTGATCC GGAGCTTTTC TTTTTTTGCC GATTAAGAAT TAATTCGGTC GAAAAAAGAA

     1801 AAGGAGAGGG CCAAGAGGGA GGGCATTGGT GACTATTGAG CACGTGAGTA TACGTGATTA

     1861 AGCACACAAA GGCAGCTTGG AGTATGTCTG TTATTAATTT CACAGGTAGT TCTGGTCCAT

     1921 TGGTGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA GATTCCGATG

     1981 CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC CCGGTTATTG

     2041 CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT CCGAAATACT

     2101 TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC AATGATTACG

     2161 GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA GAGTTCCTCG

     2221 GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA CTCAGTGCAG

     2281 CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT GGGACAGGTG

     2341 AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC CCCGAAAGCT

     2401 TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG CTTAGATTAA

     2461 ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA AAAGACTCTA

     2521 ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG TAGTATTTAT

     2581 TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC AGATGCGTAA

     2641 GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA

     2701 TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA

     2761 ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT

     2821 ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC

     2881 ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA

     2941 TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC

     3001 GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT

     3061 CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCGCG

     3121 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT

     3181 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG

     3241 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTCGT CCAAGCTTTC GCGAGCTCGA

     3301 TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT CTTTTCTGGC AACCAAACCC

     3361 ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA ACATGTAGGT GGCGGAGGGG

     3421 AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG CTAAACAAGA CTACACCAAT

     3481 TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC TGTAGCCCTA GACTTGATAG

     3541 CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG CCATCTATTG AAGTAATAAT

     3601 AGGCGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC

     3661 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA

     3721 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC

     3781 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT

     3841 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA

     3901 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT

     3961 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCATA TGTGAACAAG

     4021 TTTGTACAAA AAAGCAGGCT CTCCAACCAC CATGAATTCT ATACGAAGTT ATTAGGTCTA

     4081 GAGATCTGTT TAGCTTGCCT CGTCCCCGCC GGGTCACCCG GCCAGCGACA TGGAGGCCCA

     4141 GAATACCCTC CTTGACAGTC TTGACGTGCG CAGCTCAGGG GCATGATGTG ACTGTCGCCC

     4201 GTACATTTAG CCCATACATC CCCATGTATA ATCATTTGCA TCCATACATT TTGATGGCCG

     4261 CACGGCGCGA AGCAAAAATT ACGGCTCCTC GCTGCAGACC TGCGAGCAGG GAAACGCTCC

     4321 CCTCACAGAC GCGTTGAATT GTCCCCACGC CGCGCCCCTG TAGAGAAATA TAAAAGGTTA

     4381 GGATTTGCCA CTGAGGTTCT TCTTTCATAT ACTTCCTTTT AAAATCTTGC TAGGATACAG

     4441 TTCTCACATC ACATCCGAAC ATAAACAACC ATGGGTAAGG AAAAGACTCA CGTTTCGAGG

     4501 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT

     4561 GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG

     4621 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA

     4681 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT

     4741 GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA

     4801 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT

     4861 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG

     4921 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC

     4981 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA

     5041 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA

     5101 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA

     5161 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT

     5221 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA

     5281 TCAGTACTGA CAATAAAAAG ATTCTTGTTT TCAAGAACTT GTCATTTGTA TAGTTTTTTT

     5341 ATATTGTAGT TGTTCTATTT TAATCAAATG TTAGCGTGAT TTATATTTTT TTTCGCCTCG

     5401 ACATCATCTG CCCAGATGCG AAGTTAAGTG CGCAGAAAGT AATATCATGC GTCAATCGTA

     5461 TGTGAATGCT GGTCGCTATA CTGCTGTCGA TTCGATACTA ACGCCGCCAT CCAGTGTCGA

     5521 AAACGAGCTC CCGGGTACCC AGCTTTCTTG TACAAAGTGG TTGGTGGTGG CGGATCCGGT

     5581 GGAGGTGGAT CAATGCAGAT TTTCGTCAAG ACTTTGACCG GTAAAACCGG AACATTGGAA

     5641 GTTGAATCTT CCGATACCAT CGACAACGTT AAGTCGAAAA TTCAAGACAA GGAAGGAATC

     5701 CCTGGTTACC CATACGATGT TCCTGACTAT GCGGGCTATC CGTATGACGT CCCGGACTAT

     5761 GCAGGATCCT ATCCATATGA CGTTCCAGAT TACGCTGCTC AGTGCTGAGG CGCGCCACTT

     5821 CTAAATAAGC GAATTTCTTA TGATTTATGA TTTTTATTAT TAAATAAGTT ATAAAAAAAA

     5881 TAAGTGTATA CAAATTTTAA AGTGACTCTT AGGTTTTAAA ACGAAAATTC TTATTCTTGA

     5941 GTAACTCTTT CCTGTAGGTC AGGTTGCTTT CTCAGGTATA GTATGAGGTC GCTCTTATTG

     6001 ACCACACCTC TACCGGCAGC ATGCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT

     6061 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA

     6121 AGCCTGGGGT GCCTAATGAG TGAGGTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC

     6181 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GGATTAATGA ATCGGCCAAC GCGCGGGGAG

     6241 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT

     6301 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA

     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG

     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA

     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT

     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT

     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT

     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC

     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT

     6781 ATCGCCACTG GCAGCAGCCA CTAGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC

     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT

     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA

     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA

     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA

     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT

     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA

     7201 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC

     7261 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG

     7321 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT

     7381 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT

     7441 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG

     7501 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC

     7561 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA

     7621 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC

     7681 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT

     7741 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG

     7801 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT

     7861 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG

     7921 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC

     7981 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC

     8041 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA

     8101 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG

     8161 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCT

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